Research and use of spot human ELISA kit

1. Composition of human ELISA kit 1. Precision test plate Microtest Plates 96 wells 2. Enzyme Conjugate 12.0ml3, Standard 5 x 1.0ml 4. Colorant A, Substrate A 6.0ml5 Toner B, Substrate B 6.0ml 6, Stop Solution 6.0ml 7, concentrated washing solution (1:20) Rinsing Buffer 60.0ml 8, 5 times the sample dilution solution 15.0m Second, the precautions for this human ELISA kit is In vitro testing reagents, used within the validity period, the reagents should be regarded as infectious agents, and reagents with different batch numbers should not be mixed. Before use, the reagents in the box should be taken out and left at room temperature for at least 30 minutes. Store at 2-8 ° C, do not freeze, please see the label in the box for the expiration date. After the concentrated washing solution crystallizes, please incubate at 37 ° C for 15 minutes. 3. Operation steps Add 100 ul of the diluted sample and standard respectively to each well. The plate was incubated at 37 ° C for 30 minutes. Shake off the liquid in the plate, wash with the application washing liquid, stay for 30 seconds each time, pat dry on the paper. Repeat the operation 5 times. Add 100ul of enzyme-labeled coupling solution to each well. The plate was incubated at 37 ° C for 30 minutes. Shake off the liquid in the plate, wash with the application washing liquid, stay for 30 seconds each time, pat dry on the paper. The human ELISA kit was repeated 5 times. Add 50 ul each of Substrate A and Substrate B to each well, and place in a 37 ° C incubator for 15 minutes. 8. Add 50ul of stop solution to each well and read the OD value at 450nm. 4. Result judgment instrument value: Read the OD value of each well on a microplate reader with a wavelength of 450nm. Detection value range: 0 – 80ng / ml Sensitivity: 0.5ng / ml BC029-500ml AME'S 500ml BC032-500ml TC-100 500ml CLS1128-200ml cell separation solution 1.128 200ml CLS1068-1-200ml cell separation solution 1.068-1 200ml CLS1071-200ml cell separation solution 1.071 200ml CLS1063-200ml cell separation solution 1.063 200ml CLS1068-200ml cell separation solution 1.068 200ml CLS1082-200ml cell separation solution 1.082 200ml CLS1093-200ml cell separation solution 1.093 200ml

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